Sugarcane Genes Associated to Sucrose Content

Supplemental Tables

Additional file 1: Table 2 - Brix degree and sugar content of progenies. Brix degree, sucrose, glucose and fructose were determined from 10-month old plants of progeny 1 and 11-month old plants of progeny 2. The measurements were made from juice extracted from the 9th internode.

Additional file 2: Table 4 - SAS showing differential expression when high and low Brix plants were compared or when mature and immature internodes were compared using cDNA microarrays. The table also shows differential expression of the same SAS as seen in [32] for plants submitted to drought and ABA treatment. The table lists a SAS whose expression was enriched or decreased as determined by the Outliers Search Method in two technical replicates for each biological sample. The expression ratio for each technical replicate is in brackets.

Additional file 3: Table 5 - P value of qRT-PCR. Genes associated with sucrose content, drought, ABA and sugars were validated by qRT-PCR. The tables indicate all the genes evaluated and the values of P for differential expression.

Additional file 4: Table 6 - Sugarcane and Arabidopsis orthologues similarly regulated by sucrose and glucose.

Additional file 5: Figure 7 - Inferred phylogenetic relationships among tblastx hits using the sugarcane SAS as queries. The amino acid alignments were performed with ClustalX. The distances were obtained by p-distance and topography inferred with Neighbor-Joining (NJ) using only the aligned blocks (complete deletion). Analysis were conducted in MEGA4. The continuous blocks show regulation by sucrose and the pointed blocks show regulation by glucose (in both cases red for induction and green for repression). A - SCRFLR2037F09.g (Calreticulin 2); B - SCEQRT1024E12.g (Phenylalanine ammonia-lyase); C - SCCCRZ1001G10.g (IAA16); D - SCACLR2007G02.g and SCRFLR1034G06.g (canePKABA1-1 and canePKABA1-3); E - SCQGLR1085F11.g (Dehydrin). The sequences names correspond to those present in the protein data sets showed in Material & Methods: AT – Arabidopsis thaliana; Gm – Glycine max (soybean); jgi|Poptr1 – Populus trichocarpa; LOC Os – Oryza sativa (rice); Sb – SAorghum bicolorjgi|Selmo1 – Selaginella moellendorffii; jgi|Phypa1_1 – Physcomitrella patens patens; jgi|MicpuC2 – Micromonas pusilla CCMP1545, jgi|MicpuN2 – Micromonas strain RCC299; jgi|Volca1 – Volvox carteri; jgi|Chlre3 – Chlamydomonas reinhardtii. (sorghum);