Elevated CO2 in Sugarcane


Supplementary Material


Table S1: Differentially expressed genes selected for real-time PCR validation.
Primers were designed using Primer Express 2.0 software (ABI Biosystems). BLAST searches against the SUCEST database were conducted to ensure the specificity of the selected primers. The specificity of the PCR products were confirmed by dissociation curves. The P values were calculated in function of the microarray expression. For a description of the P calculation, refer to the Material and Methods section.

Table S2: Expression patterns of the SUCEST-FUN chip components.
The table indexes the log2 ratios of the microarray signal between the two channels (Cy3 and Cy5). M=log2 ratio of the microarray signal intensity. Only the differentially expressed SAS were confirmed by re-sequencing.

Outliers data

C1xE1 13 weeks: png intervals
C1xE1 22 weeks: png intervals
C2xE2 13 weeks: png intervals
E1xC1 13 weeks: png intervals
E2xC2 13 weeks: png intervals
E2xC2 22 weeks: png intervals

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